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Calling all scientists, preferably biochemists - I have a problem associated with a report I have to have done and handed in.
Unfortunately due to my poor motivation I was unable to attend the class where it was explained thus I am completely bedazzled.
The problem is to do with cytometry. More specifically, the cell cycle. It involves a technique called Pulse-Labelled Mitoses. Radiolabelling thymidine, and looking for radiolabelled mitotic cells. The outcome is a sine-wave style graph, from which parameters of the cell-cycle can be established. Does ANYONE know how to get these parameters from this graph? Please help me!
Unfortunately due to my poor motivation I was unable to attend the class where it was explained thus I am completely bedazzled.
The problem is to do with cytometry. More specifically, the cell cycle. It involves a technique called Pulse-Labelled Mitoses. Radiolabelling thymidine, and looking for radiolabelled mitotic cells. The outcome is a sine-wave style graph, from which parameters of the cell-cycle can be established. Does ANYONE know how to get these parameters from this graph? Please help me!
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Shouldn't you refer to a specialist forum?
You know, one for biochemists?
You know, one for biochemists?
I do 'anal' electronics at Uni!
Goatboy wrote:
> Hooplah (mate and guitarist) is an analytical research chemist.
Huh huh - you said 'anal' - huhhuh huh.
> Hooplah (mate and guitarist) is an analytical research chemist.
Huh huh - you said 'anal' - huhhuh huh.
Franny wrote:
> The outcome is a sine-wave style graph, from which parameters of the
> cell-cycle can be established. Does ANYONE know how to get these
> parameters from this graph?
Hmmm, my biology and chemistry knowledge only goes up to A-level, so I can`t be any help on that side of it, but reading a sine curve is one thing I should be able to do.
If you are just trying to express the curve in terms of a sine wave, and find the coefficients and other parameters, then thats easy, just look at the intersection between the curve and the x-axis.
Basic rules:
Sin(2x) - The 2, or whatever coefficient you put there will cause the curve to stretch or compact in line with the x-axis.
2Sin(x) - In this case, the 2 will cause the graph to stretch along the y-axis.
Sin(x+q) - The q will correspond to an entire shift of the graph along the x-axis
Sin(x) + q - This time the q moves it in the y-axis.
I`m guessing all this is probably no help as its basic GCSE stuff, and I`m sure you`re trying to find out more advanced behavioural patterns, but there you go just in case.
> The outcome is a sine-wave style graph, from which parameters of the
> cell-cycle can be established. Does ANYONE know how to get these
> parameters from this graph?
Hmmm, my biology and chemistry knowledge only goes up to A-level, so I can`t be any help on that side of it, but reading a sine curve is one thing I should be able to do.
If you are just trying to express the curve in terms of a sine wave, and find the coefficients and other parameters, then thats easy, just look at the intersection between the curve and the x-axis.
Basic rules:
Sin(2x) - The 2, or whatever coefficient you put there will cause the curve to stretch or compact in line with the x-axis.
2Sin(x) - In this case, the 2 will cause the graph to stretch along the y-axis.
Sin(x+q) - The q will correspond to an entire shift of the graph along the x-axis
Sin(x) + q - This time the q moves it in the y-axis.
I`m guessing all this is probably no help as its basic GCSE stuff, and I`m sure you`re trying to find out more advanced behavioural patterns, but there you go just in case.
Hooplah (mate and guitarist) is an analytical research chemist.
But yes, do your own homework.
But yes, do your own homework.
Do your own homework Franny!
agent under fire wrote:
it will be easy to persuade me.
Cool, vote Little Hedgehog, as she's a great story writer, and she has a GSOH. Vote for her, please!!
it will be easy to persuade me.
Cool, vote Little Hedgehog, as she's a great story writer, and she has a GSOH. Vote for her, please!!
*Is surprised KR wasn't scared off by multi-sybillic words*
All i know about ssxpro is that he started th elongest thread in ps1 and ps2 forum and i never see him post in it! Why you voting for him? I have no one else in mind so it will be easy to persuade me.
thats why you should vote for ssxpro as notable :D
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